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KMID : 1189420100230020125
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2010 Volume.23 No. 2 p.125 ~ p.138
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Anti-inflammatory effects of the water extract of Ecliptae Herba
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Jo Hee-Chang
Jung Ho-Jun
Kim Sang-Chan
Jee Seon-Young
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Abstract
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Objective : The present study was examined to evaluate the effects of Ecliptae Herba on the production of inflammatory mediators in vivo and in vitro.
Methods : In cell viability, all three doses of Ecliptae Herba extract (25, 50 and 100 μg/㎖) had no significant cytotoxicity during the experimental period. The increases of NO production and iNOS expression were detected in LPS-activated cells compared to control, but these increases were dose-dependently attenuated by pre-treatment with Ecliptae Herba extract.
Results :
1. LPS plays a pivotal roles in inducing to the massive production of pro-inflammatory cytokines such as TNF-α, IL-1β and IL-6 in macrophages.
2. Ecliptae Herba extract reduced the elevated production of cytokines by LPS.
3. Ecliptae Herba extract reduced PGE2 levels in a dose-dependent manner as a consequence of inhibition of COX-2 activity.
4. Ecliptae Herba extract significantly reduced the nuclear translocation of NF-κB induced by LPS.
5. In histopathological study, Ecliptae Herba effectively inhibited the increases of hind paw edema, skin thicknesses and inflammatory cell infiltrations induced by carrageenan treatment.
Conclusions : These results provide evidences that therapeutic effect of Ecliptae Herba on the acute inflammation is partly due to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2 through the suppression of NF-κB.
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KEYWORD
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Ecliptae Herba, Anti-inflammaory effects, NO, PGE2, iNOS, COX-2, NFκB, TNF-α, IL-1β, IL-6
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